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Identification of novel peptide antagonists for GPIIb/IIIa from a conformationally constrained phage peptide library
Author(s) -
O'Neil Karyn T.,
Hoess Ronald H.,
Jackson Sharon A.,
Ramachandran Narayana Swamy,
Mousa Shaker A.,
DeGrado William F.
Publication year - 1992
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.340140411
Subject(s) - cyclic peptide , peptide , peptide library , chemistry , phage display , cysteine , stereochemistry , disulfide bond , combinatorial chemistry , residue (chemistry) , biochemistry , peptide sequence , enzyme , gene
Methods have recently been developed to present vast libraries of random peptides on the surface of filamentous phage. To introduce a degree of conformational constraint into random peptides, a library of hexapeptides flanked by cysteine residues (capable of forming cyclic disulfides) was constructed. This library was screened using the platelet glycoprotein, IIb/IIIa, which mediates the aggregation of platelets through binding of fibrinogen. A variety of peptides containing the sequence Arg‐Gly‐Asp or Lys‐Gly‐Asp were discovered and synthesized. The cyclic, disulfide bonded forms of the peptides bound IIb/IIIa with dissociation constants in the nanomolar range, while reduced forms or an analogue in which Ser replaced the Cys residues bound considerably less tightly. These results demonstrate the feasibility for introducing conformational constraints into random peptide libraries and also demonstrates the potential for using phage peptide libraries to discover pharmacologically active lead compounds. © 1992 Wiley‐Liss, Inc.