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Crystallization and preliminary X‐ray diffraction analysis of staphylococcal enterotoxin type C
Author(s) -
Bohach Gregory A.,
Chi YoungIn,
Stauffacher Cynthia V.
Publication year - 1992
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.340130208
Subject(s) - triclinic crystal system , crystallization , tetragonal crystal system , ammonium sulfate , enterotoxin , crystallography , polyethylene glycol , chemistry , molecule , crystal structure , materials science , escherichia coli , chromatography , organic chemistry , biochemistry , gene
Abstract The Type C staphylococcal enterotoxin produced by Staphylococcus aureus strain FRI‐909 has been crystallized using a combination of two precipitants, ammonium sulfate and polyethylene glycol 400, with the addition of small amounts of detergent. Two related crystal forms have been obtained, one triclinic, and one tetragonal, both with one toxin molecule per asymmetric unit. These crystals are stable for at least 75 hr in the X‐ray beam and diffract to at least 2.2 and 2.6 Å, respectively. The triclinic crystals have unit cell parameters a = 38.5 Å, b = 43.7 Å, c = 36.9 Å, and interaxial angles α = 99.9°, β = 95.8°, and γ = 98.5°. The tetragonal crystals are of space group P 4 1 22 with unit cell parameters a = 43.4 Å and c = 278.0 Å. © 1992 Wiley‐Liss, Inc.