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Single crystals of bacteriophage T7 RNA polymerase
Author(s) -
Sousa Rui,
Rose John P.,
Je Chung Yong,
Lafer Eileen M.,
Wang BiCheng
Publication year - 1989
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.340050403
Subject(s) - rna polymerase , polymerase , t7 rna polymerase , monoclinic crystal system , crystallography , resolution (logic) , rna , bacteriophage , dna , chemistry , microbiology and biotechnology , biology , crystal structure , biochemistry , escherichia coli , gene , artificial intelligence , computer science
Abstract Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 × 0.3 × 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which in enzymatically active upon dissolution. These crystals belong to the monoclinic space group P 2 1 and have unit cell parameters a =114.5 Å, b =139.6 Å, c =125.7 Å, β=98.1° Self‐rotation function studies indicate that there are three molecules per asymmetricunit. The crystals diffract to at least 3.0 Å resolution. These are the first crystals of a DNA‐dependent RNA polymerase suitable for high‐resolution X‐ray structure determination.