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Comparative proteome analysis reveals pathogen specific outer membrane proteins of Leptospira
Author(s) -
Dhandapani Gunasekaran,
Sikha Thoduvayil,
Rana Aarti,
Brahma Rahul,
Akhter Yusuf,
Gopalakrishnan Madanan Madathiparambil
Publication year - 2018
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.25505
Subject(s) - leptospira , leptospira interrogans , proteome , biology , bacterial outer membrane , microbiology and biotechnology , pathogen , computational biology , serotype , genetics , gene , escherichia coli
Proteomes of pathogenic Leptospira interrogans and L. borgpetersenii and the saprophytic L. biflexa were filtered through computational tools to identify Outer Membrane Proteins (OMPs) that satisfy the required biophysical parameters for their presence on the outer membrane. A total of 133, 130, and 144 OMPs were identified in L. interrogans , L. borgpetersenii , and L. biflexa , respectively, which forms approximately 4% of proteomes. A holistic analysis of transporting and pathogenic characteristics of OMPs together with Clusters of Orthologous Groups (COGs) among the OMPs and their distribution across 3 species was made and put forward a set of 21 candidate OMPs specific to pathogenic leptospires. It is also found that proteins homologous to the candidate OMPs were also present in other pathogenic species of leptospires. Six OMPs from L. interrogans and 2 from L. borgpetersenii observed to have similar COGs while those were not found in any intermediate or saprophytic forms. These OMPs appears to have role in infection and pathogenesis and useful for anti‐leptospiral strategies.