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The conserved Arg241‐Glu439 salt bridge determines flexibility of the inositol 1,4,5‐trisphosphate receptor binding core in the ligand‐free state
Author(s) -
Ida Yoichi,
Kidera Akinori
Publication year - 2013
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.24304
Subject(s) - salt bridge , inositol , molecular dynamics , crystallography , chemistry , biophysics , inositol phosphate , inositol trisphosphate receptor , hinge , binding site , stereochemistry , receptor , physics , biology , mutant , biochemistry , computational chemistry , gene , classical mechanics
Inositol 1,4,5‐trisphosphate receptor (InsP 3 R) is an intracellular Ca 2+ ‐release channel activated by binding of inositol 1,4,5‐trisphosphate (InsP 3 ) to the InsP 3 binding core (IBC). Structural change in the IBC upon InsP 3 binding is the key process in channel pore opening. In this study, we performed molecular dynamics (MD) simulations of the InsP 3 ‐free form of the IBC, starting with removal of InsP 3 from the InsP 3 ‐bound crystal structure, and obtained the structural ensemble of the InsP 3 ‐free form of the IBC. The simulation revealed that the two domains of the IBC largely fluctuate around the average structure with the hinge angle opened 17° more than in the InsP 3 ‐bound form, and the twist angle rotated by 45°, forming interdomain contacts that are different from those in the bound form. The InsP 3 binding loop was disordered. The InsP 3 ‐free form thus obtained was reproduced four times in simulations started from a fully extended configuration of the two domains. Simulations beginning with the fully extended form indicated that formation of a salt bridge between Arg241 and Glu439 is crucial for stabilizing the closed form of the two domains. Mutation of Arg241 to Gln prevented formation of the compact structure by the two domains, but the fully flexible domain arrangement was maintained. Thus, the Arg241‐Glu439 salt bridge determines the flexibility of the InsP 3 ‐free form of the IBC.Proteins 2013; 81:1699–1708. © 2013 Wiley Periodicals, Inc.