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Structural and biochemical characterization of native and recombinant single insulin‐like growth factor‐binding domain protein (SIBD‐1) from the Central American Hunting Spider Cupiennius salei (Ctenidae)
Author(s) -
Trachsel Christian,
Widmer Christine,
Kämpfer Urs,
Bühr Claudia,
Baumann Tommy,
KuhnNentwig Lucia,
Schürch Stefan,
Schaller Johann,
Baumann Ulrich
Publication year - 2012
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.24119
Subject(s) - spider , recombinant dna , biology , microbiology and biotechnology , insulin like growth factor binding protein , chemistry , biochemistry , growth factor , insulin like growth factor , receptor , gene , zoology
Cupiennius salei single insulin‐like growth factor binding domain protein (SIBD‐1) is an 8.6 kDa Cys‐, Pro‐, and Gly‐rich protein, discovered in the hemocytes of the Central American hunting spider Cupiennius salei . SIBD‐1 exhibits high sequence similarity to the N‐terminal domain of the insulin‐like growth factor‐binding protein superfamily and has been reported to play an important role in the spider's immune system. Here, the recombinant expression and the elucidation of the three‐dimensional structure of recombinant SIBD‐1 and the characterization of the sugar moiety at Thr2 of native SIBD‐1 is described in detail. Proteins 2012; © 2012 Wiley Periodicals, Inc.