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Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata
Author(s) -
Sampathkumar Parthasarathy,
Kim Seung Joong,
Manglicmot Danalyn,
Bain Kevin T.,
Gilmore Jeremiah,
Gheyi Tarun,
Phillips Jeremy,
Pieper Ursula,
FernandezMartinez Javier,
Franke Josef D.,
Matsui Tsutomu,
Tsuruta Hiro,
Atwell Shane,
Thompson Devon A.,
Emtage J. Spencer,
Wasserman Stephen R.,
Rout Michael P.,
Sali Andrej,
Sauder J. Michael,
Almo Steven C.,
Burley Stephen K.
Publication year - 2012
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.24102
Subject(s) - nucleoporin , nuclear pore , saccharomyces cerevisiae , yeast , candida glabrata , cytoplasm , nuclear transport , biology , homology modeling , microbiology and biotechnology , chemistry , biochemistry , biophysics , genetics , cell nucleus , candida albicans , enzyme
The nuclear pore complex (NPC), embedded in the nuclear envelope, is a large, dynamic molecular assembly that facilitates exchange of macromolecules between the nucleus and the cytoplasm. The yeast NPC is an eightfold symmetric annular structure composed of ∼456 polypeptide chains contributed by ∼30 distinct proteins termed nucleoporins. Nup116, identified only in fungi, plays a central role in both protein import and mRNA export through the NPC. Nup116 is a modular protein with N‐terminal “FG” repeats containing a Gle2p‐binding sequence motif and a NPC targeting domain at its C‐terminus. We report the crystal structure of the NPC targeting domain of Candida glabrata Nup116, consisting of residues 882–1034 [CgNup116(882–1034)], at 1.94 Å resolution. The X‐ray structure of CgNup116(882–1034) is consistent with the molecular envelope determined in solution by small‐angle X‐ray scattering. Structural similarities of CgNup116(882–1034) with homologous domains from Saccharomyces cerevisiae Nup116, S. cerevisiae Nup145N, and human Nup98 are discussed. Proteins 2012; © 2012 Wiley Periodicals, Inc.