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Analysis on folding of misgurin using two‐dimensional HP model
Author(s) -
Yan Shaomin,
Wu Guang
Publication year - 2012
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.23233
Subject(s) - amino acid , folding (dsp implementation) , chemistry , glycine , native state , peptide , protein folding , stereochemistry , crystallography , biochemistry , electrical engineering , engineering
Misgurin is an antimicrobial peptide from the loach, while the hydrophobic‐polar (HP) model is a way to study the folding conformations and native states in peptide and protein although several amino acids cannot be classified either hydrophobic or polar. Practically, the HP model requires extremely intensive computations, thus it has yet to be used widely. In this study, we use the two‐dimensional HP model to analyze all possible folding conformations and native states of misgurin with conversion of natural amino acids according to the normalized amino acid hydrophobicity index as well as the shortest benchmark HP sequence. The results show that the conversion of misgurin into HP sequence with glycine as hydrophobic amino acid at pH 2 has 1212 folding conformations with the same native state of minimal energy –6; the conversion of glycine as polar amino acid at pH 2 has 13,386 folding conformations with three native states of minimal energy –5; the conversion of glycine as hydrophobic amino acid at pH 7 has 2538 folding conformations with three native states of minimal energy –5; and the conversion of glycine as polar amino acid at pH 7 has 12,852 folding conformations with three native states of minimal energy –4. Those native states can be ranked according to the normalized amino acid hydrophobicity index. The detailed discussions suggest two ways to modify misgurin. Proteins 2011. © 2012 Wiley Periodicals, Inc.