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In‐cell protein NMR and protein leakage
Author(s) -
Barnes Christopher O.,
Pielak Gary J.
Publication year - 2011
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.22906
Subject(s) - leakage (economics) , nuclear magnetic resonance spectroscopy , cell , escherichia coli , nuclear magnetic resonance , chemistry , biophysics , protein expression , microbiology and biotechnology , biochemistry , biology , physics , gene , economics , macroeconomics
In‐cell nuclear magnetic resonance spectroscopy is a tool for studying proteins under physiologically relevant conditions. In some instances, however, protein signals from leaked protein are observed in the liquid surrounding the cells. Here, we examine the expression of four proteins in Escherichia coli . We describe the controls that should be used for in‐cell NMR experiments and show that leakage is likely when the protein being studied exceeds ∼20% of the total cellular protein. Proteins 2011. © 2010 Wiley‐Liss, Inc.