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Crystal structure of a novel Sm‐like protein of putative cyanophage origin at 2.60 Å resolution
Author(s) -
Das Debanu,
Kozbial Piotr,
Axelrod Herbert L.,
Miller Mitchell D.,
McMullan Daniel,
Krishna S. Sri,
Abdubek Polat,
Acosta Claire,
Astakhova Tamara,
Burra Prasad,
Carlton Dennis,
Chen Connie,
Chiu HsiuJu,
Clayton Thomas,
Deller Marc C.,
Duan Lian,
Elias Ylva,
Elsliger MarcAndré,
Ernst Dustin,
Farr Carol,
Feuerhelm Julie,
Grzechnik Anna,
Grzechnik Slawomir K.,
Hale Joanna,
Han Gye Won,
Jaroszewski Lukasz,
Jin Kevin K.,
Johnson Hope A.,
Klock Heath E.,
Knuth Mark W.,
Kumar Abhinav,
Marciano David,
Morse Andrew T.,
Murphy Kevin D.,
Nigoghossian Edward,
Nopakun Amanda,
Okach Linda,
Oommachen Silvya,
Paulsen Jessica,
Puckett Christina,
Reyes Ron,
Rife Christopher L.,
Sefcovic Natasha,
Sudek Sebastian,
Tien Henry,
Trame Christine,
Trout Christina V.,
van den Bedem Henry,
Weekes Dana,
White Aprilfawn,
Xu Qingping,
Hodgson Keith O.,
Wooley John,
Deacon Ashley M.,
Godzik Adam,
Lesley Scott A.,
Wilson Ian A.
Publication year - 2009
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.22360
Subject(s) - nucleic acid , rna , biology , computational biology , dna , genomic dna , escherichia coli , genetics , similarity (geometry) , sequence alignment , gene , peptide sequence , biochemistry , computer science , artificial intelligence , image (mathematics)
Abstract ECX21941 represents a very large family (over 600 members) of novel, ocean metagenome‐specific proteins identified by clustering of the dataset from the Global Ocean Sampling expedition. The crystal structure of ECX21941 reveals unexpected similarity to Sm/LSm proteins, which are important RNA‐binding proteins, despite no detectable sequence similarity. The ECX21941 protein assembles as a homopentamer in solution and in the crystal structure when expressed in Escherichia coli and represents the first pentameric structure for this Sm/LSm family of proteins, although the actual oligomeric form in vivo is currently not known. The genomic neighborhood analysis of ECX21941 and its homologs combined with sequence similarity searches suggest a cyanophage origin for this protein. The specific functions of members of this family are unknown, but our structure analysis of ECX21941 indicates nucleic acid‐binding capabilities and suggests a role in RNA and/or DNA processing. Proteins 2009. © 2008 Wiley‐Liss, Inc.