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Structure of Xanthomonas axonopodis pv. citri YaeQ reveals a new compact protein fold built around a variation of the PD‐(D/E)XK nuclease motif
Author(s) -
Guzzo Cristiane R.,
Nagem Ronaldo A. P.,
Barbosa João A. R. G.,
Farah Chuck S.
Publication year - 2007
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.21556
Subject(s) - nuclease , motif (music) , biology , fold (higher order function) , genetics , microbiology and biotechnology , dna , physics , computer science , acoustics , programming language
The YaeQ family of proteins are found in many Gram‐negative and a few Gram‐positive bacteria. We have determined the first structure of a member of the YaeQ family by X‐ray crystallography. Comparisons with other structures indicate that YaeQ represents a new compact protein fold built around a variation of the PD‐(D/E)XK nuclease motif found in type II endonucleases and enzymes involved in DNA replication, repair, and recombination. We show that catalytically important residues in the PD‐(D/E)XK nuclease superfamily are spatially conserved in YaeQ and other highly conserved YaeQ residues may be poised to interact with nucleic acid structures. Proteins 2007. © 2007 Wiley‐Liss, Inc.