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A ToxR‐based dominant‐negative system to investigate heterotypic transmembrane domain interactions
Author(s) -
Lindner Eric,
Langosch Dieter
Publication year - 2006
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.21226
Subject(s) - transmembrane protein , transmembrane domain , microbiology and biotechnology , protein–protein interaction , mutant , activator (genetics) , biology , transcription factor , transcription (linguistics) , biophysics , gene , chemistry , genetics , receptor , linguistics , philosophy
The ToxR transcription activator system is a genetic tool widely used to examine homotypic interactions of α‐helical transmembrane domains that drive or support oligomerization of integral membrane proteins. Here, we present a variant of this system that was developed to investigate heterotypic interactions. In this system, a homotypically interacting transmembrane domain within the ToxR protein is coexpressed with a potential competitor that is embedded within an inactivated ToxR mutant. Successful heterotypic interaction competes with homotypic interaction in a dominant‐negative fashion as revealed by reduced reporter gene transcription. We expect that this system will be useful for the characterization of transmembrane domains whose homotypic and heterotypic interactions compete with each other. Proteins 2006. © 2006 Wiley‐Liss, Inc.