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NMR structure and binding studies confirm that PA4608 from Pseudomonas aeruginosa is a PilZ domain and a c‐di‐GMP binding protein
Author(s) -
Ramelot Theresa A.,
Yee Adelinda,
Cort John R.,
Semesi Anthony,
Arrowsmith Cheryl H.,
Kennedy Michael A.
Publication year - 2006
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.21199
Subject(s) - guanosine , biochemistry , signal transducing adaptor protein , binding protein , binding site , protein structure , guanosine monophosphate , chemistry , binding domain , biology , stereochemistry , nucleotide , receptor , gene
PA4608 is a 125 residue protein from Pseudomonas aeruginosa with a recent identification as a PilZ domain and putative bis‐(3′‐5′)‐cyclic dimeric guanosine monophosphate (c‐di‐GMP) adaptor protein that plays a role in bacterial second‐messenger regulated processes. The nuclear magnetic resonance (NMR) structure of PA4608 has been determined and c‐di‐GMP binding has been confirmed by NMR titration studies. The monomeric core structure of PA4608 contains a six‐stranded anti‐parallel β barrel flanked by three helices. Conserved surface residues among PA4608 homologs suggest the c‐di‐GMP binding site is at one end of the barrel and includes residues in the helices as well as in the unstructured N‐terminus. Chemical shift changes in PA4608 resonances upon titration with c‐di‐GMP confirm binding. This evidence supports the hypothesis that proteins containing PilZ domains are the long‐sought c‐di‐GMP adaptor proteins. Proteins 2007. © 2006 Wiley‐Liss, Inc.