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Intrinsic unstructuredness and abundance of PEST motifs in eukaryotic proteomes
Author(s) -
Singh Gajinder Pal,
Ganapathi Mythily,
Sandhu Kuljeet Singh,
Dash Debasis
Publication year - 2005
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.20746
Subject(s) - pest analysis , abundance (ecology) , biology , proteome , evolutionary biology , computational biology , ecology , botany , genetics
The study of unfolded protein regions has gained importance because of their prevalence and important roles in various cellular functions. These regions have characteristically high net charge and low hydrophobicity. The amino acid sequence determines the intrinsic unstructuredness of a region and, therefore, efforts are ongoing to delineate the sequence motifs, which might contribute to protein disorder. We find that PEST motifs are enriched in the characterized disordered regions as compared with globular ones. Analysis of representative PDB chains revealed very few structures containing PEST sequences and the majority of them lacked regular secondary structure. A proteome‐wide study in completely sequenced eukaryotes with predicted unfolded and folded proteins shows that PEST proteins make up a large fraction of unfolded dataset as compared with the folded proteins. Our data also reveal the prevalence of PEST proteins in eukaryotic proteomes (∼25%). Functional classification of the PEST‐containing proteins shows an over‐ and under‐representation in proteins involved in regulation and metabolism, respectively. Furthermore, our analysis shows that predicted PEST regions do not exhibit any preference to be localized in the C terminals of proteins, as reported earlier. Proteins 2006. © 2005 Wiley‐Liss, Inc.