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Solution structure of two insect‐specific spider toxins and their pharmacological interaction with the insect voltage‐gated Na + channel
Author(s) -
Ferrat G.,
Bosmans F.,
Tytgat J.,
Pimentel C.,
Chagot B.,
Gilles N.,
Nakajima T.,
Darbon H.,
Corzo G.
Publication year - 2005
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.20424
Subject(s) - sodium channel , cockroach , biophysics , venom , electrophysiology , insect , xenopus , chemistry , stereochemistry , biology , biochemistry , sodium , organic chemistry , neuroscience , gene , ecology
δ‐PaluIT1 and δ‐paluIT2 are toxins purified from the venom of the spider Paracoelotes luctuosus . Similar in sequence to μ‐agatoxins from Agelenopsis aperta , their pharmacological target is the voltage‐gated insect sodium channel, of which they alter the inactivation properties in a way similar to α‐scorpion toxins, but they bind on site 4 in a way similar to β‐scorpion toxins. We determined the solution structure of the two toxins by use of two‐dimensional nuclear magnetic resonance (NMR) techniques followed by distance geometry and molecular dynamics. The structures of δ‐paluIT1 and δ‐paluIT2 belong to the inhibitory cystine knot structural family, i.e. a compact disulfide‐bonded core from which four loops emerge. δ‐PaluIT1 and δ‐paluIT2 contain respectively two‐ and three‐stranded anti‐parallel β‐sheets as unique secondary structure. We compare the structure and the electrostatic anisotropy of those peptides to other sodium and calcium channel toxins, analyze the topological juxtaposition of key functional residues, and conclude that the recognition of insect voltage‐gated sodium channels by these toxins involves the β‐sheet, in addition to loops I and IV. Besides the position of culprit residues on the molecular surface, difference in dipolar moment orientation is another determinant of receptor binding and biological activity differences. We also demonstrate by electrophysiological experiments on the cloned insect voltage‐gated sodium channel, para , heterologuously co‐expressed with the tipE subunit in Xenopus laevis oocytes, that δ‐paluIT1 and δ‐paluIT2 procure an increase of Na + current. δ‐PaluIT1‐OH seems to have less effect when the same concentrations are used. Proteins 2005. © 2005 Wiley‐Liss, Inc.