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Premium Registering α‐helices and β‐strands using backbone CH…O interactions
Singh S. Kumar,
Babu M. Madan,
Balaram P.
Publication year2003
Publication title
proteins: structure, function, and bioinformatics
Resource typeJournals
PublisherWiley Subscription Services
Abstract The possible occurrence of a novel helix terminating structural motif in proteins involving a stabilizing short CH…O interaction has been examined using a dataset of 634 non‐homologous protein structures (≤2.0 Å). The search for this motif was prompted by the crystallographic characterization of a novel structural feature in crystals of a synthetic decapeptide in which extension of a Schellman motif led to the formation of a CH…O hydrogen bond between the T‐4 C α H and the T+1 CO groups, where T is the helix terminator adopting a left handed (α L ) conformation. More than 100 such motifs with backbone conformation superposing well with the peptide examples were identified. In several examples, formation of this motif led to an approximately antiparallel arrangement of a helical segment with an extended β‐strand. Careful examination of these examples suggested the possibility of registering antiparallel arrangement of helices and strands by means of backbone CH…O interactions with a regular periodicity. Model building resulted in the generation of idealized αβ and βα motifs, which can then be generalized to higher‐order repetitive structures. Inspection of the antiparallel αβ motif revealed a significant propensity for Ser, Glu, and Gln residues at the T‐4 position resulting in further stabilization using an O…HN side‐chain–backbone hydrogen bond. Modeling studies revealed ready accommodation of serine residues along the helix face that contacts the strand. The theoretically generated folds correspond to “open” polypeptide structures. Proteins 2003;51:167–171. © 2003 Wiley‐Liss, Inc.
Subject(s)antiparallel (mathematics) , biochemistry , chemistry , crystallography , hydrogen bond , magnetic field , molecule , organic chemistry , physics , protein structure , quantum mechanics , stereochemistry , structural motif
SCImago Journal Rank1.699

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