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Mapping pathways of allosteric communication in GroEL by analysis of correlated mutations
Author(s) -
Kass Itamar,
Horovitz Am
Publication year - 2002
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.10180
Subject(s) - groel , chaperonin , allosteric regulation , groes , cooperativity , context (archaeology) , adenosine triphosphate , cooperative binding , biology , chemistry , protein folding , biochemistry , genetics , biophysics , binding site , enzyme , gene , escherichia coli , paleontology
An interesting example of an allosteric protein is the chaperonin GroEL. It undergoes adenosine 5′‐triphosphate‐induced conformational changes that are reflected in binding of adenosine 5′‐triphosphate with positive cooperativity within rings and negative cooperativity between rings. Herein, correlated mutations in chaperonins are analyzed to unravel routes of allosteric communication in GroEL and in its complex with its co‐chaperonin GroES. It is shown that analysis of correlated mutations in the chaperonin family can provide information about pathways of allosteric communication within GroEL and between GroEL and GroES. The results are discussed in the context of available structural, genetic, and biochemical data concerning short‐ and long‐range interactions in the GroE system. Proteins 2002;48:611–617. © 2002 Wiley‐Liss, Inc.