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A clade of trypsins found in cold‐adapted fish
Author(s) -
Roach Jared C.
Publication year - 2002
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/prot.10062
Subject(s) - clade , phylogenetic tree , psychrophile , biology , mesophile , enzyme , computational biology , biochemistry , genetics , gene , bacteria
Abstract A clade of trypsins, known as group III, is identified by phylogenetic analysis. These trypsins occur in fish that spend all or part of their lives at temperatures near 0°C and may represent extreme psychrophilic enzymes. A principal component analysis of amino acid compositions distinguishes group III from mesophilic trypsins, as do molecular trees and multidimensional scaling of molecular sequence distances. The primary sequences of group III trypsins, in conjunction with the known structures of mesophilic trypsins, permit insight into function and mechanisms of cold adaption. The techniques employed are broadly applicable to phylogenies characterized by a markedly different, or “fast‐evolving,” clade. An updated lactate dehydrogenase molecular tree illustrates an additional fast‐evolving clade. Proteins 2002;47:31–44. © 2002 Wiley‐Liss, Inc.