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Retinoblastoma gene mutations in primary human prostate cancer
Author(s) -
Kubota Yoshinobu,
Fujinami Kiyoshi,
Uemura Hiroji,
Dobashi Yasushi,
Miyamoto Hiroshi,
Iwasaki Yoshiko,
Kitamura Hitoshi,
Shuin Taro
Publication year - 1995
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990270604
Subject(s) - prostate cancer , retinoblastoma , prostate , medicine , gene , cancer research , mutation , oncology , biology , cancer , pathology , genetics
Structural alterations in the entire coding regions (exons 1 to 27) of the retinoblastoma (RB) gene in primary human prostate cancers were investigated, using polymerase chain reaction and single strand conformational polymorphism analysis of RNA. Of 25 samples obtained from patients, four (16.4%) were found to have RB alterations. DNA sequencing of the PCR products revealed point mutations resulting in single amino‐acid substitutions of exons 6 and 19 in two cases, and base deletions of exons 8 and 17 in two cases. Two of four cases with RB mutations were moderately differentiated localized tumors and other two with RB mutations were poorly differentiated tumors with metastases. Our results suggest that RB gene mutation is involved in progression steps of prostate carcinogenesis. © 1995 Wiley‐Liss, Inc.