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Expression of estrogen receptor in diseased human prostate assessed by non‐radioactive in situ hybridization and immunohistochemistry
Author(s) -
Ehara Hidetoshi,
Koji Takehiko,
Deguchi Takashi,
Yoshii Akira,
Nakano Masahiro,
Nakane Paul K.,
Kawada Yukimichi
Publication year - 1995
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990270603
Subject(s) - in situ hybridization , stromal cell , estrogen receptor , immunohistochemistry , hyperplasia , androgen receptor , estrogen , riboprobe , estrogen receptor alpha , biology , prostate , estrogen receptor beta , pathology , androgen , endocrinology , medicine , messenger rna , cancer research , prostate cancer , cancer , breast cancer , gene , hormone , biochemistry
To understand the role of estrogen in the pathogenesis of benign prostatic hyperplasia, expressions of estrogen receptor (ER) mRNA and ER protein by in situ hybridization and by immunohistochemistry, respectively, were investigated in human prostatic tissues. In non‐malignant region, ER mRNA and ER protein were found in cytoplasm and nucleus, respectively, of stromal cells, but not in glandular epithelial and basal cells. In benign regions, ER mRNA/ER protein positive cells were found in fibromyoadenomatous and myoadenomatous hyperplasia, but not in adenomatous hyperplasia. A striking feature was periacinar arrangement of ER mRNA/ER protein positive stromal cells in all prostate carcinoma treated with androgen withdrawal. The ER mRNA/ER protein positive cells were immunohistochemically identified as fibroblasts, myoblasts, and smooth muscle cells. These results indicate that stromal cells are the primary target of estrogen in prostate, and that androgen withdrawal upregulates the expression of ER gene. © 1995 Wiley‐Liss, Inc.