z-logo
Premium
Influence of dihydrotestosterone, epidermal growth factor, and basic fibroblast growth factor on the cell kinetics of the PC3, DUI45, and LNCap prostatic cancer cell lines: Relationship with DNA ploidy level
Author(s) -
Janssen Thierry,
Kiss Robert,
Dedecker Robert,
Petein Michel,
Pasteels JeanLambert,
Schulman Claude
Publication year - 1995
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990270507
Subject(s) - du145 , lncap , biology , epidermal growth factor , cell culture , cell cycle , basic fibroblast growth factor , cell growth , endocrinology , medicine , cell , microbiology and biotechnology , growth factor , cancer research , cancer cell , cancer , biochemistry , genetics , receptor
The cell kinetics (percentage of cells in the S + G2 phases of the cell cycle) and the DNA ploidy levels (nuclear DNA content) were determined in 108 samples each of the PC3, DU145, and LNCaP prostate cancer models. This was carried out by means of the digital cell image analysis of Feulgen‐stained nuclei. Two to three hundred cell nuclei were analyzed for each of the 324 samples under study. The three cell lines were submitted to experimental conditions including the addition of dihydrotestosterone (DHT), epidermal growth factor (EGF), and basic fibroblast growth factor (bFGF), either alone or in combination, to the culture media. The results show that under the present culture conditions, the PC3 cell line was DHT‐, EGF‐ and bFGF‐insensitive. In contrast to what is generally reported in the literature, the DU145 cell line was DHT‐ and EGF‐sensitive under the present culture conditions, but bFGF‐insensitive. The LNCaP cell line was DHT‐sensitive, but EGF‐ and bFGF‐insensitive. While mainly tetraploid, the three cell lines nevertheless exhibited a significant level of heterogeneity in their nuclear DNA content distributions. Indeed, the proportions of non‐tetraploid (diploid, hyperdiploid, triploid, hypertriploid, hypertetraploid, polymorphic) DNA histograms were 14% in the PC3, 16% in the DU145, and 29% in the LNCaP cell lines. These results suggest that the DNA ploidy level would not influence the hormone sensitivity level in the cell lines since they had significantly distinct hormone sensitivity profiles while remaining mainly tetraploid. © 1995 Wiley‐Liss, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here