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Survival of human prostate carcinoma, benign hyperplastic prostate tissues, and IL‐2‐activated lymphocytes in SCID mice
Author(s) -
Lubaroff David M.,
Cohen Michael B.,
Shultz Leonard D.,
Beamer Wesley G.
Publication year - 1995
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990270107
Subject(s) - prostate , androgen , prostate cancer , pathology , adenocarcinoma , severe combined immunodeficiency , medicine , biology , cancer , in vivo , cancer research , hormone , microbiology and biotechnology
Mice, homozygous for the mutation severe combined immunodeficiency ( scid ) and also segregating for the mutation hypogonadal ( hpg ), were tested for their potential use as an in vivo model system for studying the growth of human prostate cancer and benign hyperplastic prostate tissue grafts. Fresh human prostate cancer or benign hyperplastic prostate tissue was implanted subcutaneously into androgen‐replete C.B. 17 scid/scid males, and into androgen‐deficient hpg/hpg scid/scid or androgen‐replete +/? scid scid males. The tissue grafts grew in both androgen‐replete and androgen‐deficient host mice. When dihydrotestosterone (DHT) was administered at tissue grafting, both the incidence and size of the tissue grafts increased. Histology of tissue from tumors in the androgen‐replete hosts showed a well‐differentiated prostate adenocarcinoma, whereas the androgen‐deficient hpg/hpg scid/scid host showed either undifferentiated tumors or adenocarcinomas with few glandular structures. These data suggest the androgen deficient environment selected for growth of androgen‐independent tumor tissue. Finally, when interleukin‐2 (IL‐2)‐activated tumor‐infiltrating lymphocytes were injected into scid/scid hosts, the cells were found to survive and could be identified in the spleen of the recipient mice. These results indicate that growth of human prostate tissues and IL‐2‐activated lymphocytes in scid/scid mice is a viable model system for in vivo studies of prostatic disease. © 1995 Wiley‐Liss, Inc.