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Expression of cellular adhesion molecules on human prostate tumor cell lines
Author(s) -
Rokhlin Oskar W.,
Cohen Michael B.
Publication year - 1995
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990260406
Subject(s) - lncap , cell culture , extracellular matrix , cell adhesion molecule , integrin , metastasis , cancer research , cell sorting , cell adhesion , prostate cancer , biology , prostate , cell , laminin , microbiology and biotechnology , flow cytometry , cancer , genetics
By flow cytometric analysis we have examined the expression of cellular adhesion molecules (CAMs) on the surface of four different human prostate tumor cell lines: DU 145, from a brain metastasis; PC 3, from a bone metastasis; LNCaP.FGC, from a lymph node metastasis; and a primary tumor cell line, ND 1. The corresponding ligands for the expressed CAMs were, by and large, extracellular matrix proteins. We detected high‐level expression of ICAM‐1 on three of the four prostate cell lines, whereas LNCaP cells were negative. We observed unstable, heterogeneous expression of E‐cadherin in the cell lines DU 145, PC 3, and ND 1. Flow cytometric cell sorting enabled us to divide PC 3 cells into negative and bright positive subpopulations but, after several cell divisions in culture, sorted cells returned to the original heterogeneous phenotype. The laminin‐specific α 6 β 4 integrin was not expressed by LNCaP, and was expressed at a low level and heterogeneously on DU 145 and PC 3 cell lines. In contrast, ND 1 cells, derived from a primary tumor, showed homogeneous and high‐level expression of the α 6 β 4 integrin. All of the prostate cell lines expressed the RGD‐dependent binding of α 3 β 1 and α 5 β 1 integrins and did not reveal non‐RGD‐dependent α 4 β 1 integrin expression. This finding provides a stimulus to investigate the inhibition capacity of RGD‐containing peptides on the metastatic behavior of prostate tumor cells.