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IGF‐Binding proteins in human prostate tumor cells: Expression and regulation by 1,25‐dihydroxyvitamin D 3
Author(s) -
Drivdahl Rolf H.,
Loop Stephen M.,
Andress Dennis L.,
Ostenson Richard C.
Publication year - 1995
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990260203
Subject(s) - insulin like growth factor binding protein , endocrinology , prostate , messenger rna , medicine , cell culture , growth factor , biology , binding protein , stimulation , cell growth , cancer research , insulin like growth factor , chemistry , gene , receptor , cancer , biochemistry , genetics
The expression of the six known insulin‐like growth factor binding proteins (IGFBPs) and their corresponding messenger RNAs has been examined in three cell lines established from surgical and biopsy specimens of human prostate carcinoma. All three cell lines produced both IGFBP‐4 and IGFBP‐6 and the respective mRNAs; expression of IGFBP‐6 has not been previously demonstrated in human prostate tumor cells. No other binding proteins were detected. The levels of IGFBP mRNAs were not regulated by androgens or IGF‐1, but the level of IGFBP‐6 mRNA was sharply increased by 1,25‐dihydroxyvitamin D 3 (1,25(OH)D 3 ). The stimulation was dose‐dependent with a maximum effect at 10 nM 1,25(OH)D 3 and a clearly discernible effect at 0.1 nM. The results support a role for vitamin D in the control of prostate tumor growth, mediated at least in part by interaction with IGFs and specific IGFBPs.