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Growth, morphology, and morphometry of human hypertrophic prostate cells treated with suramin in vitro
Author(s) -
De Angeli Sergio,
Fandella Andrea,
Conconi Maria Teresa,
Anselmo Giuseppe,
Parnigotto Pier Paolo
Publication year - 1994
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990250302
Subject(s) - suramin , in vitro , cell growth , biology , cell culture , growth inhibition , microbiology and biotechnology , endocrinology , medicine , chemistry , biochemistry , genetics
Abstract This work studies the effects of suramin on the growth and morphology of cell strain U285, obtained from human prostate hypertrophic tissue and cultured in vitro. The FRAME cytotoxicity test was performed to evaluate the inhibition of growth induced by suramin. Cells were exposed to suramin at the time of seeding and 24 hours later; neutral red was added with and without suramin. An optical microscope connected to a computer‐aided system and a scanning electron microscope were used to study morphological changes induced by suramin. Growth inhibition depends on drug concentration and exposure period. Moreover, the effect of suramin on neutral red uptake is reversible. Suramin 1,000 μM causes the cells to become spheroid, and they fail to form a monolayer. Our data indicate that the addition of suramin during the lag phase decreases the rate of cell proliferation. © 1994 Wiley‐Liss, Inc.