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Detection of prostatic‐inhibin‐like peptide in the cytoplasm of LNCaP cells, a human prostatic adenocarcinoma cell line
Author(s) -
Hurkadli Kalpana S.,
Sheth Anil R.,
Lokeshwar Balakrishna,
Block Norman L.
Publication year - 1994
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990240603
Subject(s) - lncap , cell culture , immunocytochemistry , biology , intracellular , peptide , cytoplasm , endocrinology , medicine , prostate , microbiology and biotechnology , biochemistry , cancer , genetics
Prostatic inhibin peptide (PIP) is a 94‐amino acid peptide involved in various cellular functions. The concentration of this peptide changes with prostatic pathophysiology suggesting a role in various disease conditions; present study was undertaken to investigate the presence of this peptide in two human prostatic cell lines: LNCaP and PC3 cells. The LNCaP cells showed an intense intracellular fluorescence pattern after staining with rabbit‐anti‐PIP antiserum and FITC conjugated goat antirabbit‐IgG, while the PC3 cells did not exhibit any fluorescence. There was no alteration in the concentration of PIP in LNCaP cells with or without supplementation of steroids in culture medium. Immunoblot analysis indicates similarities between PIP from LNCaP cells and that from the human seminal plasma. Thus, present study demonstrates the presence of PIP in a human prostatic cell line, i.e., LNCaP cells. Its intracellular concentration is androgen independent, and has a close similarity with PIP isolated from the human seminal plasma. © 1994 Wiley‐Liss, Inc.