Premium
Vitamin A regulates proliferation and differentiation of human prostatic epithelial cells
Author(s) -
Peehl Donna M.,
Wong Stephen T.,
Stamey Thomas A.
Publication year - 1993
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990230107
Subject(s) - prostate , epithelium , vitamin , cell growth , endocrinology , cellular differentiation , biology , medicine , microbiology and biotechnology , cancer research , pathology , gene , cancer , biochemistry
The response of cultured human prostatic epithelial cells to vitamin A was measured by clonal growth assay in serum‐free medium. Retinoic acid at 3 nM or higher inhibited the proliferation of cell strains derived from normal, benign hyperplastic and malignant tissues, while lower levels (0.03 nM) were stimulatory. Reduced proliferation induced by retinoic acid was accompanied by a marked change in morphology, as intercellular adhesion decreased. In conjunction, the expression of keratins 8 and 18, associated with the differentiated luminal phenotype of prostatic epithelia, was increased. In post‐confluent cultures, retinoic acid prevented the appearance of keratin 1, which accompanied the development of a squamous phenotype by cells maintained under these conditions. The findings of this study indicate a role for vitamin A as a modulator of the growth and differentiation of prostatic epithelial cells. © 1993 Wiley‐Liss, Inc.