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Effects of prolactin on explant cultures of rat ventral prostate: Morphological and immunohistochemical study
Author(s) -
Romero L.,
Muñoz C.,
Lopez A.,
Vilches J.
Publication year - 1993
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990220102
Subject(s) - explant culture , prolactin , immunohistochemistry , prostate , monoclonal antibody , keratin , epithelium , biology , pathology , medicine , endocrinology , antibody , in vitro , hormone , immunology , biochemistry , cancer
Specimens derived from rat ventral prostate were cultured by an explant culture technique using differents concentrations of ovine prolactin. Sacrified explants embedded on paraffin were sectioned for morphologic and immunocytochemical studies using antibodies against prostatic acid phosphatase, prostatic specific antigen, and wide‐spectrum monoclonal keratin. Prolactin significantly stimulated the growth of these cells in the concentration range of 1 × 10 −2 ui/ml, but was inhibitory at a concentration of 1 and 0.1 ui/ml. The 1 × 10 −3 and 1 × 10 −4 ui/ml prolactin concentrations demonstrated the preservation of a glandular epithelium with a columnar shape, similar to the normal appearance of ventral prostate from rats. © 1993 Wiley‐Liss, Inc.