Premium
Relationship of proliferating cell nuclear antigen (PCNA) in prostatic carcinomas to various clinical parameters
Author(s) -
Harper M. E.,
GlynneJones E.,
Goddard L.,
Wilson D. W.,
Griffiths K.,
Conn I. G.,
Matenhelia S. S.,
Peeling W. B.
Publication year - 1992
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990200309
Subject(s) - proliferating cell nuclear antigen , pathology , medicine , prostate , carcinoma , antigen , immunohistochemistry , cancer , immunology
Proliferating cell nuclear antigen (PCNA) expression was determined immunohistochemically, using a monoclonal antibody PC10, in 102 prostatic carcinoma samples and in prostate tissue from 21 patients with benign prostatic hyperplasis (BPH). The percentage of cells with stained nuclei ranged from 1% to 58% in the carcinoma specimens and 0% to 10% in the BPH specimens. A semiquantitative scoring system was devised for the degree of PCNA positivity observed in the tumors. Statistical analysis of the PCNA score in relation to the histological grade of the tumors gave a significant positive or negative correlation between these parameters P <0.001. No significant correlation between PCNA score was, however, seen with metastatic status, T category (TMN classification) of the primary tumor, or the patient's age at diagnosis. In 65 prostatic cancer patients of known survival, those individuals whose tumors had a PCNA score of ± (< 10% of nuclei stained) were compared with those patients whose tumors were either 1+, 2+, or 3+ (>10% of nuclei stained). Life table analysis of the two groups indicated that the patients with the lower PCNA score survived significantly longer than those with the higher PCNA scores, P <0.04. Comparison of the Ki‐67 expression in frozen sections with the PCNA expression in wax‐embedded tissue of 86 prostatic carcinomas was also undertaken. A significant correlation between these two parameters was found, P <0.001, although the growth fraction estimated by Ki‐67 expression was generally lower than that given by the PCNA scoring system. 1992 Wiley‐Liss, Inc.