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Interactions between epidermal growth factor‐mediated autocrine regulation and linoleic acid‐stimulated growth of a human prostate cancer cell line
Author(s) -
Connolly Jeanne M.,
Rose David P.
Publication year - 1992
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990200210
Subject(s) - du145 , autocrine signalling , epidermal growth factor , cell growth , growth factor , biology , receptor , endocrinology , cell culture , linoleic acid , medicine , microbiology and biotechnology , cancer cell , biochemistry , fatty acid , cancer , genetics , lncap
Human prostate cancer (PC) cell lines possess epidermal growth factor (EGF) receptors and secrete EGF‐related polypeptides. We used an EGF receptor‐blocking antibody (anti‐EGF.R) to demonstrate a functional autocrine loop, as well as the interaction between this and the effects of linoleic acid (LA), an omega‐6 fatty acid, on PC cell growth. The anti‐EGF.R competed effectively with [ 125 I]EGF for receptors on DU145 PC cells, and on a high‐passage DU145 variant (DU145M); when added to the culture medium, it suppressed both DU145 and DU145M cell growth in a dose‐dependent manner. LA, a precursor for eicosanoid synthesis, had little effect on DU145 cell growth rate but stimulated DU145M growth in a concentration‐related manner over a range of 0.25–2.0 μg/ml. Anti‐EGF.R (10 −9 M) caused suppression of LA‐stimulated growth of DU145M cells in serum‐free medium, which was prevented by the addition of 2 nM EGF. We conclude that an EGF.R‐mediated autocrine loop is involved in PC cell growth regulation and that at least one site of action may be the synthesis of eicosanoids from their LA precursor.