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Autocrine regulation of DU145 human prostate cancer cell growth by epidermal growth factor‐related polypeptides
Author(s) -
Connolly Jeanne M.,
Rose David P.
Publication year - 1991
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990190210
Subject(s) - autocrine signalling , du145 , epidermal growth factor , growth factor , biology , cell growth , receptor , endocrinology , epidermal growth factor receptor , cell culture , fetal bovine serum , tgf alpha , medicine , cancer cell , cell , cancer , biochemistry , lncap , genetics
The DU145 human prostate cancer cell line possesses epidermal growth factor (EGF) receptors and synthesizes both EGF and the related polypeptide transforming growth factor α (TGF‐α). A monoclonal antibody to the EGF receptor was used to determine whether these characteristics were indicative of a functional autocrine regulatory system. This antibody competed effectively with [ 125 I]EGF for binding to DU145 cell binding sites over a 1 × 10 −11 to 1 × 10 −7 M concentration range, and did so with a capability similar to that of the two natural ligands. It inhibited growth of these cells in both 3% fetal bovine serum‐supplemented and serum‐free medium; in experiments with incubation times of 3–5 days there was a 45–50% reduction in cell number. Growth suppression by the EGF receptor blockade of cells plated at a density of 1.5 × 10 4 cells/ml/well was reversed competitively by the addition of EGF to the medium; 0.3 nM completely eliminated the inhibitory effect of a 1 × 10 −9 M antibody concentration. It is concluded that DU145 cell growth is regulated by an EGF‐mediated autocrine loop.