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Expression of endogenous receptors for neoglycoproteins in dunning R3327 rat prostatic carcinoma
Author(s) -
Sinowatz F.,
Gabius H.J.,
Hellmann K. P.,
Amselgruber W.,
Schneider M. R.
Publication year - 1990
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990160208
Subject(s) - polyclonal antibodies , lectin , receptor , endogeny , basal (medicine) , immunocytochemistry , biology , prostate , antibody , endocrinology , medicine , cancer , pathology , microbiology and biotechnology , cancer research , immunology , biochemistry , insulin
An important animal model for human prostatic adenocarcinoma is the Dunning R3327 rat carcinoma. In the present study this tumor was further characterized by analyzing the expression of endogenous sugar‐binding proteins using glycohistochemistry and immuno‐cytochemistry as well as affinity chromatography and gel electrophoresis. Our glycohis‐tochemical and glycobiochemical results provide evidence for the presence of specific receptors for various carbohydrate moieties. Remarkably, basal cells of the Dunning tumor contain an endogenous lectin with specificity for β‐galactosides that is not found in basal cells of the normal rat prostate. This finding was corroborated using polyclonal antibodies against an immunologically related β‐galactoside‐specific lectin from bovine heart. Basal cells of prostatic carcinoma may therefore behave different from normal basal cells. This difference could have a significant impact on the development of prostatic cancer.