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Evidence for two aspartate transport systems in prostate epithelial cells
Author(s) -
Franklin Renty B.,
Lao Lixing,
Costello L. C.
Publication year - 1990
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990160205
Subject(s) - intracellular , extracellular , compartment (ship) , distribution (mathematics) , prostate , chemistry , biophysics , washout , kinetics , biochemistry , endocrinology , biology , medicine , mathematical analysis , oceanography , physics , mathematics , cancer , quantum mechanics , geology
Abstract The transport of aspartate was measured in isolated prostate cells. Two kinetically distinct uptake systems of high (K m = 10.0 μM) and low (K m = 0.8 mM) affinity were observed. Transport by both systems was Na + dependent and saturable. The tissue distribution of aspartate was also determined by measuring the washout kinetics of endogenous and radio‐labeled aspartate from prostate fragments. The distribution of aspartate resulted in 47% of aspartate in the intracellular compartment and 52% in the extracellular (presumably) lu‐menal compartment. The distribution of aspartate resulted in a calculated intracellular to plasma concentration gradient of approximately 43. We concluded that prostate cells maintain a considerable intracellular to plasma gradient for aspartate and that these cells contain a transport mechanism capable of uptake of aspartate against this considerable gradient.