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Immunocytochemical demonstration of insulin or insulin‐like immunoreactivity in the rat prostate gland
Author(s) -
Stahler Michael S.,
Pansky Ben,
Budd G. Colin
Publication year - 1988
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990130209
Subject(s) - immunostaining , insulin , epithelium , immunocytochemistry , prostate , endocrinology , medicine , antiserum , biology , cytoplasm , immunohistochemistry , pathology , antibody , microbiology and biotechnology , immunology , cancer
The indirect immunocytochemical technique was used in conjunction with anti‐insulin antisera to localize insulin‐like immunoreactivity in tissue sections and primary cell cultures from rat prostate gland. Positive immunostaining for insulin‐like reactivity was demonstrated in the epithelium of the prostate gland. There appeared to be some variation in the intensity and localization of the immunoreactivity within different regions of the gland. Prostatic epithelial cells grown in culture in an insulin‐free medium displayed strong cytoplasmic immunostaining when treated with anti‐insulin antisera, while nuclear staining was absent. These results demonstrate that insulin‐like immunoreactivity is present in the epithelium of the prostate gland and suggest that there may be some local insulin or insulin‐like synthesis in this organ.