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Application of octadecylsilyl‐silica in purification studies related to the nontransformed rat ventral prostate androgen receptor
Author(s) -
Koutsilieris Michael,
Bouthillier François,
Grondin Francine,
Radwan Farouk,
Carmel Michel,
Elhilali Mostafa,
Lehoux JeanGuy
Publication year - 1988
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990130207
Subject(s) - androgen receptor , androgen , chromatography , receptor , prostate , biology , gel permeation chromatography , endocrinology , medicine , chemistry , biochemistry , prostate cancer , hormone , organic chemistry , cancer , polymer
We investigated the application of octadecylsilyl (ODs)‐silica in studies related to characterization and purification of the nontransformed rat ventral prostate androgen receptor. The results indicated that ODS‐silica successfully separates the free [ 3 H]R1881 from the labeled [ 3 H]R1881 transformed (4‐5s) and nontransformed (8‐9s) rat ventral prostate androgen receptors. Partial purification of the 8‐9s receptor form was performed by the fractionation of rat cytosol using cartridges of the ODS‐silica and fast‐flow‐rate phosphocellulose chromatography. Further purification was accomplished by differential chromatography (DEAE‐ cellulose and slow‐flow‐rate phosphocellulose chromatography). This partially purified 8‐9s receptor, when analyzed on a gel permeation high‐performance liquid chromatography column, resulted in a three‐peak pattern of UV absorbance. One of these peaks corresponded to a 59‐kD non[ 3 H]R1881‐binding protein and the remaining two corresponded to 270‐kD and 190‐kD[ 3 H]R1881‐binding proteins. These results demonstrate the usefulness of ODS‐silica in androgen receptor studies. The association of a 59‐kD nonsteroid binding protein with the nontransformed rat ventral prostate androgen receptor is discussed.