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Androgens stimulate both growth rate and epidermal growth factor receptor activity of the human prostate tumor cell LNCaP
Author(s) -
Schuurmans Alex L. G.,
Bolt Joan,
Mulder Eppo
Publication year - 1988
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990120108
Subject(s) - lncap , androgen receptor , epidermal growth factor , endocrinology , medicine , androgen , receptor , cell growth , biology , prostate , chemistry , cancer research , prostate cancer , hormone , biochemistry , cancer
LNCaP cells (derived from a lymph node carcinoma of the human prostate) contain androgen receptors and show androgen‐responsive growth in vitro. Maximal effects on growth were seen at 0.1 nM of the synthetic androgen R1881 or 0.2 nM of epidermal growth factor (EGF); both compounds independently increased the growth rate 2–3 times. EGF‐receptors were measured after 6 days culture in the presence or absence of 0.1 nM R1881. A 2.3–fold increase in receptor number/cell was found when binding was measured at 0°C (from 12,500 to 28,900 sites/cell in stimulated cells). The kD value (0.45 nM) was not affected by androgen treatment. The increase of EGF‐receptor activity was first observed between 6 and 12 h after exposure to androgen. It is concluded that LNCaP cells are sensitive to low concentrations of EGF (or EGF‐like compounds) and that one of the mechanisms involved in androgen action on these cells is an increase of EGF‐receptor expression at the cell surface.