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Cytochemistry and biochemistry of acid phosphatases VII: Immunohistochemistry of canine prostatic acid phosphatase
Author(s) -
Aumüller Gerhard,
Vedder Helmut,
EnderleSchmitt Ulrike,
Seitz Jürgen
Publication year - 1987
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990110102
Subject(s) - acid phosphatase , antiserum , prostatic acid phosphatase , fast protein liquid chromatography , phosphatase , cytochemistry , biochemistry , biology , microbiology and biotechnology , chemistry , immunocytochemistry , isoelectric focusing , antigen , enzyme , endocrinology , immunology
Acid phosphatase (E.C. 3.1.3.2.) has been isolated from canine prostatic gland homogenates by gel permeation chromatography (AcA34 or G150), by affinity chromatography (con A‐Sepharose), or by using fluid phase liquid chromatography (FPLC) using Superose 12 and Mono P columns. Acid phosphatase‐enriched fractions were submitted to analytical SDS‐PAGE or to analytical isolelecuic focusing. A protein with a molecular weight of 30 kD (on SDS gels) was used for immunization of rabbits. The antiserum produced was cross‐reactive with prostatic acid phosphatase (canine and human) as shown by immunoblotting. When applied to paraffin or plastic sections of normal canine prostate, a positive immunoreaction was found exclusively in the secretory cells. In experimentally altered glands (castration and/or hormone treatment), a varying pattern of immunoreactive cells was found. In canine prostatic carcinomas, intensively reacting cell clusters were found along with nonreactive cells. The antiserum was also slightly cross‐reactive with the respective human antigen, but the cross‐reactivity of an antiserum prepared against human prostatic secretory acid phosphatase with canine prostatic acid phosphatase was far more pronounced.