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Monoclonal antibody specific to acid phosphatase isoenzyme 4
Author(s) -
Dang Howard,
Lam KwokWai,
Li ChinYang,
Wold Lester E.,
Sun Tsieh,
Yam Lung T.
Publication year - 1986
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990090108
Subject(s) - monoclonal antibody , isozyme , microbiology and biotechnology , radioimmunoassay , acid phosphatase , immunohistochemistry , antibody , western blot , biology , staining , biochemistry , chemistry , blot , enzyme , immunology , genetics , gene
Prostatic acid phosphatase isoenzyme 4 was purified by ion exchange column chromatography, followed by high pressure liquid chromatography. The highly purified enzyme was used to produce monoclonal antibody from immunized BALB/c mice. The antibody was specific to isoenzyme 4, with negligible affinity to isoenzyme 2. The specificity of the monoclonal antibody was evaluated by Western blot analysis and by inhibition of radioimmunoassay. Immunohistochemistry method using the antibody to isoenzyme 2 showed heavy staining on the cell surface in contrast to the even staining throughout the cytoplasm when monoclonal anti‐isoenzyme 4 was used. These results reflect the secretory nature of isoenzyme 2 and the non‐secretory nature of isoenzyme 4.