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A search for differentiation markers in prostatic fluid components with two‐dimensional electrophoresis and multiple protein detectors
Author(s) -
Johnson A. H.,
Merchant D. J.,
Norman J.,
Bourgeois N.,
Lee C.
Publication year - 1985
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990070411
Subject(s) - silver stain , stain , heterologous , electrophoresis , polyacrylamide gel electrophoresis , gel electrophoresis , pathology , chromatography , chemistry , microbiology and biotechnology , staining , biology , medicine , biochemistry , gene , enzyme
Components of prostatic fluid resolved by two‐dimensional polyacrylamide gel electrophoresis were detected by dye, silver stain, and autoradiography. Five components identified by autoradiography were not recognized by the dye or silver stain. While labeled components were found in primary fractions of split ejaculates and seminal fluid in addition to prostatic fluid, they have not been found in tissue extracts of heterologous organs or human serum.