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Studies of androgen and estrogen binding in normal canine prostatic tissue and in epithelial and stromal cell lines derived from the canine prostate
Author(s) -
Eaton C. L.,
Hamilton T. C.,
Kenvyn K.,
Pierrepoint C. G.
Publication year - 1985
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990070406
Subject(s) - stromal cell , estrogen , androgen , diethylstilbestrol , prostate , cytosol , endocrinology , biology , dihydrotestosterone , protamine sulfate , medicine , epithelium , chemistry , hormone , protamine , biochemistry , enzyme , cancer , heparin , genetics
Androgen and estrogen binding to cytosol proteins was examined in preparations derived from canine prostatic tissue and from epithelial and fibroblastoid cell lines. Binding parameters were characterized by saturation analysis using a protamine sulfate precipitation procedure and by analysis of the mobility of steroid‐binding moieties in sucrose density gradients. Androgen‐binding studies demonstrated the presence of a single class of high‐affinity binding component (kD = 1‐2 × 10 −9 M), with specificity for 5α‐dihydrotestosterone, in cytosols derived from tissue homogenates and both cell types. In estrogen‐binding studies two discrete classes of binding component were characterized of high (kD = 5‐10 × 10 −10 M) and moderate (kD = 1‐2 × 10 −8 M) affinities. Both species were present in cytosol derived from all three sources. The high‐affinity component displayed specificity for estradiol‐17β, and the second component displayed some capacity for androgen binding. These findings are discussed with reference to the anomalous actions of some androgens in the canine prostate and in relation to the putative role of steroids in stromal‐epithelial interrelationships.