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The antigenic relatedness of proteins from human and simian prostate fluid
Author(s) -
Carter Donald B.,
Timmins James G.,
Adams Lonnie D.,
Lewis Ronald W.,
Karr James P.,
Resnick Martin I.,
Buhl Allen E.
Publication year - 1985
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990060408
Subject(s) - prostate , isoelectric point , polyclonal antibodies , antibody , prostate specific antigen , biology , antigen , beagle , isoelectric focusing , microbiology and biotechnology , chemistry , immunology , biochemistry , genetics , cancer , enzyme
Two‐dimensional electophoretic analysis of human prostate fluid reveals an abundant protein migrating to a molecular weight of 15 kD and an isoelectric point of 5.5. Polyclonal antibodies were raised specifically to microgram quantities of electrophoresed, excised, and eluted PSP15 (prostate secretory protein). Western immunoblot analysis using these antibodies showed they ot only react to PSP 15 , but cross‐react with simian prostate and human seminal fluid proteins of similar molecular weights. Two‐dimensional gel immunoblots strongly suggest that the seminal protein and PSP15 are the same, thereby providing a more accessible source of the protein. The antibody to the human PSP 15 cross‐reacted with neither prostate fluid from the ventral lobe of the rat prostate nor the prostate fluid from the beagle dog.