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Antiprostate carcinoma monoclonal antibody (D83.21) cross reacts with a membrane antigen expressed on cytomegalovirus‐transformed human fibroblasts
Author(s) -
Campbell Ann E.,
Beckett Mary L.,
Starling James J.,
Sieg Susan M.,
Wright George L.
Publication year - 1985
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990060211
Subject(s) - monoclonal antibody , antigen , antibody , epitope , human cytomegalovirus , immunofluorescence , biology , microbiology and biotechnology , virology , virus , immunology
Virological and epidemiological studies have implicated human cytomegalovirus (HCMV) as a possible etiological agent of prostate cancer. Because of the suspected associations, this laboratory tested the reactivity of a prostate‐associated monoclonal antibody with HCMV‐transformed cells. This mouse monoclonal antibody, D83.21, reacts with a membrane antigen on prostate and bladder tumor cells and does not bind to a variety of other malignant or normal cells. The results of this study indicated that the prostate‐associated antibody bound to a membrane antigen on HCMV‐transformed cells as detected by radioimmunoassay, immunofluorescence, and complemented‐dependent cytotoxicity. This cross‐reactivity appeared to be specific for HCMV‐transformed cells and did not react with HCMV‐infected cells or those transformed by other viruses. Antibody affinity chromatography, used to isolate the D83.21‐reactive protein, revealed two peptides of 60 and 28 kd on both prostate tumor and HCMV‐transformed cells. The results suggest that D83.21 reacts with a common cell surface protein expressed on HCMV‐transformed cells and urogenital tumors.