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Synthesis and evaluation of immobilized androgens for affinity chromatography in the purification of nuclear androgen receptor
Author(s) -
De Larminat MarieAnne,
Bruchovsky Nicholas,
Rennie Paul S.,
Lee Sing Ping,
Tertzakian Gerald
Publication year - 1984
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990050202
Subject(s) - agarose , androgen receptor , dihydrotestosterone , chemistry , chromatography , androgen , affinity chromatography , testosterone (patch) , endocrinology , medicine , biochemistry , biology , enzyme , hormone , prostate cancer , cancer
Seven biospecific adsorbents containing immobilized androgens were synthesized: dihydro‐testosterone‐17β‐succinyl agarose, and both the unsubstituted and the 17β‐acetyl derivatives of dihydrotestosterone‐7α‐undecanoyl agarose, testosterone‐7α‐undecanoyl agarose, and 19‐nortestosterone‐7α‐undecanoyl agarose. The retention capacities for nuclear androgen receptor were generally between 40‐80% with little variation in reproducibility; the amount of binding was greatest with dihydrotestosterone‐17β‐succinyl agarose and dihydrotestoster‐one‐17β‐acetoxy‐7α‐undecanoyl agarose. Rapid flow rates were obtained with all gels, and no tendency for decomposition was observed over a period of 1 year. Factors that affected retention included the concentration of immobilized androgen, length of the linker arm, occupation of receptor sites, interval of contact with the gel, and temperature of incubation. Chemical dissociation of androgens from androgen receptor complexes with 0.2 mM mersalyl increased the retention of receptor by dihydrotestosterone‐17β‐succinyl agarose. Two elutants showed promise for the dissociation of gel‐bound receptor: 1) 0.2 mM mersalyl in the presence of 1.5 mg/ml of ovalbumin; 2) 10% (v/v) dimethylformamide:water containing 30 μM [1,2‐ 3 H] dihydrotestosterone and 0.5 M sodium thiocyanate.