Premium
Primary explant culture: An in vitro model of the human prostate
Author(s) -
Merchant Donald J.,
Clarke S. M.,
Ives K.,
Harris S.
Publication year - 1983
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990040511
Subject(s) - explant culture , in vitro , prostate , medicine , primary culture , cell culture , biology , pathology , genetics , cancer
Human prostate tissue gave rise to essentially pure cultures of basal cells in vitro. Careful analysis by phase‐contrast microscopy and photography suggested that these cells attempt to differentiate into secretory acinar cells. This was confirmed by immunocytochemical analysis for prekeratin which is present only in basal cells in situ and for prostatic acid phosphatase, prostate‐specific antigen, and prostate fluid antigen, which are present only in secretory acinar cells in situ.