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Organ culture studies of human prostatic adenocarcinomas
Author(s) -
Mistry Damienti,
Riches Andrew,
Patrick Weaver J.
Publication year - 1983
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990040310
Subject(s) - prostate , medicine , prostate disease , pathology , prostatic disease , adenocarcinoma , organ culture , urology , biology , cancer , in vitro , biochemistry
The proliferative responses of human prostatic carcinoma have been evaluated in organ culture using [ 125 I]‐iododeoxyuridine ([ 125 I]UdR) to monitor DNA synthesis. The morphological preservation was not influenced by the addition of fetal calf serum or insulin (5 μgm/ml), transfemn (10 μgm/ml), and thyrotropin releasing hormone (10 −9 M) to the active medium. Testosterone (4 ± 10 −6 M) stimulated [ 125 I]UdR uptake, whereas diethylstilboestrol (4 ± 10 −6 M) had no direct effect on uptake. Both estramustine phosphate (4 ± 10 −6 M) and oestradiol‐17β (4 ± 10 −6 M) inhibited uptake in a similar manner. Thus while explants of human prostatic carcinoma derived from transurethrally resected specimens can be well maintained in organ culture for a few days, proliferative responses are small and difficult to measure for individual patients.