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A qualitative analysis of acidic proteins associated with regressing, growing, or dividing rat ventral prostate cells
Author(s) -
Anderson K. M.,
Baranowski J.,
Rubenstein M.,
Economou S. G.
Publication year - 1983
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990040206
Subject(s) - isoelectric point , testosterone propionate , hyperplasia , androgen , biology , isoelectric focusing , prostate , polyacrylamide gel electrophoresis , endocrinology , muscle hypertrophy , medicine , gel electrophoresis , biochemistry , enzyme , genetics , cancer , hormone
Abstract A qualitative analysis of Coomassie blue R‐250‐stained acidic proteins from normal, regressing, growing or dividing rat ventral prostate cells was performed. Four prostates from each of five groups of animals that included normal, and short (3‐day) or longer‐term (6‐day) castrates, some of which received pharmacologic amounts of testosterone propionate for 3 subsequent days to induce cellular hypertrophy or hyperplasia respectively, were solubilized. Proteins were separated in the first dimension by their isoelectric points from pI 3 to 10, and in the second according to molecular weights, using SDS polyacrylamide gel electrophoresis. Composite “normograms” of protein distribution were developed, based on four different samples from each of the five groups, and 17 out of 31 possible subsets defined. Two major conclusions resulted: 1) The physiologic state of the organ could be identified by its distribution of stained proteins, reflected in the composite “normogram” for each of the five groups and 2) twelve of the seventeen identified patterns of protein distribution were provisionally correlated with the physiologic state of the sample. Excluding the trivial category of “ubiquitous” proteins, subsets including androgen‐dependent and androgen‐inhibited proteins, putative cell number or cell type‐dependent changes, proteins associated with cellular hypertrophy, hyperplasia, and short or long‐term castration and protein synthesis that may have been affected by nonandrogen‐dependent gonadal factors could provisionally be delineated. Most of these categories would not have been evident without the use of two‐dimensional protein analysis. It will be understood that these subsets, predicated upon the apparent presence or absence of proteins detected with a stain of limited sensitivity, would probably be markedly altered if a more sensitive means of protein detection were used. However, within the sensitivity provided by the dye, these conclusions are warranted.