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Epithelial and fibroblastoid cell lines derived from the normal canine prostate. II. Cell proliferation in response to steroid hormones
Author(s) -
Eaton C. L.,
Pierrepoint C. G.
Publication year - 1982
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990030508
Subject(s) - stromal cell , cell growth , biology , endocrinology , cell culture , medicine , population , estrogen , androgen , epithelium , cell , hormone , prostate , microbiology and biotechnology , cancer research , biochemistry , cancer , genetics , environmental health
Epithelial and fibroblastoid cell lines derived from the adult canine prostate, have been used as model systems in a preliminary study of the effects of steroid hormones on isolated cell populations. The mitogenic activities of various androgens and one estrogen were assessed during logarithmic growth in replicate subcultures by measurement of population size and radiolabeled nucleotide uptake in the presence of concentration ranges of these steroids. [H 3 ]‐Leucine incorporation rate was used as an estimate of protein synthesis in the epithelial cell line during the latter phase of log growth. Of the androgens examined, elevated cell population growth rate was only demonstrated in either epithelial or fibroblastoid cell cultures supplemented with 5α‐androstane‐3α, 17β‐ and 17α‐diols at physiological concentrations. No significant qualitative differences were observed between individual prostatic cell types in their proliferative response to the androgens tested. Contrasting effects were observed in the growth rates of epithelial and fibroblastoid cell types in cultures supplemented with estradiol‐17β. This estrogen was significantly mitogenic in fibroblast cultures, but did not significantly affect epithelial proliferation at the concentrations studied. These results are reviewed with reference to current concepts of steroid action in the canine prostate. The importance of the characterization of culture conditions and procedures has been emphasized as a prerequisite to the study of cell proliferation, and potential mitogens, using cell culture models. The relevance of these findings in the consideration of stromal‐epithelial interaction, and glandular differentiation, is discussed.