Premium
Primary culture of prostatic region entoderm of human fetal sinus urogenitalis: Morphological, histochemical, and electrophoretic characteristics
Author(s) -
Bozhok Yu M.,
Dziuba L. V.
Publication year - 1982
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990030102
Subject(s) - mesenchyme , prostate , fetus , pathology , biology , primary culture , anatomy , epithelium , medicine , cell culture , pregnancy , cancer , genetics
Primary cultures of entoderm from the prostatic region of the sinus urogenitalis obtained from 9 to 12‐week‐old human fetuses were established by explantation of presumptive prostate tissue on collagen. The predominant growth of entodermal cells made it possible to obtain cultures, without use of enzymes or EDTA, which were practically free of mesenchyme. Histochemical and electrophoretic methods demonstrated certain properties of the parent presumptive prostatic entodermal cells in the cultured cells. Karyological alterations observed during the course of cultivation are described.