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A chemically defined medium for the propagation of rat prostate adenocarcinoma cells
Author(s) -
Chan ShamYuen
Publication year - 1981
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.2990020307
Subject(s) - adenocarcinoma , fetuin , transferrin , cell culture , prostate , endocrinology , medicine , chemically defined medium , dexamethasone , pathology , cancer research , biology , microbiology and biotechnology , cancer , in vitro , biochemistry , genetics , glycoprotein
Abstract A chemically defined medium (MB‐DF) has been developed for the propagation of the rat prostate adenocarcinoma cell line (PA‐III). The medium (MB‐DF) was supplemented with fetuin, insulin, transferrin, dexamethasone, and 5α‐dihydrotestosterone, but require no serum supplement. The adapted line, PA‐III,. has been grown in this serum‐free medium for 30 passages over a period of 12 months without losing the malignant or other characteristics of the parent line. When transplanted into syngeneic rats, the PA‐III, cells reconstituted the adenocarcinoma and metastasized spontaneously via ipsilateral lymphatic channels to the lungs. This cell line will be of use in further analyses of enzymes associated with biological properties of the tumor cells.