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Decreased expression of lysophosphatidylcholine (16:0/OH) in high resolution imaging mass spectrometry independently predicts biochemical recurrence after surgical treatment for prostate cancer
Author(s) -
Goto Takayuki,
Terada Naoki,
Inoue Takahiro,
Kobayashi Takashi,
Nakayama Kenji,
Okada Yoshiyuki,
Yoshikawa Takeshi,
Miyazaki Yu,
Uegaki Masayuki,
Utsunomiya Noriaki,
Makino Yuki,
Sumiyoshi Shinji,
Yamasaki Toshinari,
Kamba Tomomi,
Ogawa Osamu
Publication year - 2015
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.23088
Subject(s) - prostate cancer , prostate , prostatectomy , lysophosphatidylcholine , mass spectrometry imaging , lncap , medicine , pathology , cancer , urology , chemistry , phosphatidylcholine , mass spectrometry , biochemistry , phospholipid , chromatography , membrane
Background Human prostate cancers are highly heterogeneous, indicating a need for various novel biomarkers to predict their prognosis. Lipid metabolism affects numerous cellular processes, including cell growth, proliferation, differentiation, and motility. Direct profiling of lipids in tissue using high‐resolution matrix‐assisted laser desorption/ionization imaging mass spectrometry (HR‐MALDI‐IMS) may provide molecular details that supplement tissue morphology. Methods Prostate tissue samples were obtained from 31 patients, with localized prostate cancer who underwent radical prostatectomy. The samples were assessed by HR‐MALDI‐IMS in positive mode, with the molecules identified by tandem mass spectrometry (MS/MS). The effect of identified molecules on prostate specific antigen recurrence free survival after radical prostatectomy was determined by Cox regression analysis and by the Kaplan–Meier method. Results Thirteen molecules were found to be highly expressed in prostate tissue, with five being significantly lower in cancer tissue than in benign epithelium. MS/MS showed that these molecules were [lysophosphatidylcholine (LPC)(16:0/OH)+H] + , [LPC(16:0/OH)+Na] + , [LPC(16:0/OH)+K] + , [LPC(16:0/OH)+matrix+H] + , and [sphingomyelin (SM)(d18:1/16:0)+H] + . Reduced expression of LPC(16:0/OH) in cancer tissue was an independent predictor of biochemical recurrence after radical prostatectomy. Conclusions HR‐MALDI‐IMS showed that the expression of LPC(16:0/OH) and SM(d18:1/16:0) was lower in prostate cancer than in benign prostate epithelium. These differences in expression of phospholipids may predict prostate cancer aggressiveness, and provide new insights into lipid metabolism in prostate cancer. Prostate 75:1821–1830, 2015 . © 2015 Wiley Periodicals, Inc.